Journal: iScience

Article Title: Comparative effects of hemodialysis modalities on microparticle induction and neutrophil activation in a randomized cross-over study

doi: 10.1016/j.isci.2025.113485

Figure Lengend Snippet: Effects of post-hemodialysis microparticles from the mixed dilution online-HDF (OL-HDF) or hemodialysis with median cut-off dialyzer (MCO-HDX) on human coronary artery endothelial cells (HCAECs) The representation of carboxyfluorescein succinimidyl ester (CSFE)-stained MPs (green color) represented by CSFE fluorescent intensity with the representative immunofluorescent pictures (A and B) are demonstrated ( n = 6/group, scale bar = 10 μ m). The red color in A is zona occluden-1 (ZO-1; a tight junction molecule) and the blue color is DNA-stained by 4′,6-diamidino-2-phenylindole (DAPI). The influences of the post-dialysis MPs from OL-HDF (C) or MCO+HDX (D) with 1x10 4 (light color) or 1x10 5 (heavy color) particles/μL at 24 h after inoculation, compared with control (only media but not MPs) in the condition with or without phorbol myristate acetate (PMA, the positive control activator) on HCAECs as indicated by vascular cell adhesion molecule 1 (VCAM-1) and cell apoptosis, are shown. The effect of MPs on tight junction molecules ZO-1 is also demonstrated through the intensity score of the red color with representative immunofluorescent (E, F) ( n = 6/group, scale bar = 10 μ m). Notably, the MPs were not stained to avoid the noise from the green-fluorescent color. ∗ p < 0.05 vs. control within group; # p < 0.05 vs. same condition between groups.

Article Snippet: Primary Human Coronary Endothelial Cells (HCAECs), Female , ATCC , PCS-100-020 Lot No. 70001400.

Techniques: Staining, Control, Positive Control

Journal: bioRxiv

Article Title: Chromatin landscape and enhancer-gene interaction differences between three cardiac cell types

doi: 10.1101/2025.09.23.678180

Figure Lengend Snippet:

Article Snippet: Human coronary artery smooth muscle cells (HCASMC) and Human coronary artery endothelial cells (HCAEC) were all commercially bought from Thermo Fisher Scientific, Waltham, MA, U.S.A. (product code: C-017-5C) and the American Type Culture Collection (ATCC), Manassas, VA, U.S.A. (Product code: PCS-100-020), respectively.

Techniques: Modification